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Insights into Molecular Interactions Using Photoaffinity Labeling Coupled to Mass Spectrometry  

Résumé de la présentation:
Photoaffinity labeling coupled to mass spectrometry is a powerful approach for the characterization of interaction partners at the atomic level. These studies require careful optimization, including the strategic chemical labeling of one of the partners with an affinity purification tag and a photoreactive group. UV-irradiation of the photoprobe at a defined wavelength allows the formation of new covalent bonds with specific partners in close vicinity. Benzophenone (Bzp)-induced photo-crosslinking is commonly used for protein crosslinking. Some applications in the context of peptide interaction with biological membranes [1,2], membrane or soluble proteins [3,4] will be presented. Importantly, highly relevant information on molecular interactions can arise directly from the mass spectra when considering the specific photoreactivity of Bzp and side chemical reactions occuring in complex media. In this presentation, I will show you how MS-based approaches are highly valuable to answer complex biological questions.

References
[1] L. Bechtella et al., Structural Bases for the Involvement of Phosphatidylinositol-4,5-bisphosphate in the Internalization of the Cell-Penetrating Peptide Penetratin, ACS Chem. Biol. 17, (2022), 1427.
[2] L. Bechtella et al., Benzophenone Photoreactivity in a Lipid Bilayer To Probe Peptide/Membrane Interactions: Simple System, Complex Information, Anal. Chem. 91 (2019), 9102.
[3] E. Sachon et al., Isotope and affinity tags in photoreactive substance P analogues to identify the covalent linkage within the NK-1 receptor by MALDI-TOF analysis, Anal Chem. 75 (2003), 6536.
[4] S. Clavier et al., Photocross-linked peptide-protein complexes analysis: A comparative study of CID and ETD fragmentation modes, JASMS, 26 (2015), 1014.

Chemical Concepts from Theory and Computation

The purpose of this symposium is to foster discussions between experts from the DFT, MOT and VBT and QTAIM communities on stability, bonding, reactivity, non linear optics, molecular electronics, among others. We will also welcome experts in the merging field of artificial intelligence applied to theoretical chemistry21. We especially encourage contributions that provide a broader perspective on conceptual quantum chemistry and/or establish common ground between different theoretical/computational approaches. Our ultimate aim is to provide chemical meaning to high-level quantum computations thus filling the ever-growing gap between experimentalist and computational chemists. The workshop will take place at the Institute of Analytical Sciences 5 Rue de la Doua in Villeubanne. Villeurbanne is the biggest suburb of Lyon (the second largest city in France)