Colon Cancer Traitement using Proteins based Nanoparticles containing Curcumin as active molecule

Résumé :

The main idea of this study originated from an interest in using turmeric to treat cancer cells. Turmeric possesses chemical properties that limit its use as a drug, despite having many therapeutic properties and being one of the most important anti-cancer agents. However, poor water solubility and bioavailability in the body present challenges for its pharmaceutical applications. By utilizing nanoparticles to encapsulate curcumin, the study aimed to improve its effectiveness on cancer cells. In this study, BSA (Bovine Serum Albumin) nanoparticles were used to encapsulate curcumin for the treatment of colon cancer, with the goal of improving its solubility and stability, thus enhancing bioavailability. The nanoprecipitation method with organic media was employed to create this type of nanoparticle. Curcumin was dissolved in ethanol, and BSA in water. The BSA solution was gradually added to the curcumin solution to encapsulate the curcumin. The precipitation method, followed by solvent evaporation, was used for the concomitant encapsulation of the active ingredient (curcumin) and BSA nanoparticles. Various parameters were studied, and several ratios and concentrations were tested to determine the best characterization. Subsequently, the in vitro effects of the particles on cancer cells (using a colon cancer cell line) and normal cells were observed and compared with the results from a known chemotherapy (Cisplatin). Different concentrations of the prepared drug were also tested, and the results were compared. The results were very satisfactory and demonstrated the success of the treatment in killing cancer cells, while the effect on normal cells was not as significant as with chemical treatments.

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