Spatial Multi-Omics of the Hepatology Institute of Lyon
The Spatial Multi-Omics platform of the IHU EVEREST, located at the Institute of Analytical Sciences, represents a major advancement in multi-scale and multi-omics analysis of chronic liver diseases. By combining proteomics, metabolomics, and lipidomics on a single sample, it provides an in-depth understanding of complex biological processes at the molecular level.
https://www.ihu-hepatolyon.fr/les-plateformes-technologiques/
The platform includes a state-of-the-art instrument unique in France — the timsTOF fleX MALDI-2 mass spectrometer from Bruker — enabling MALDI imaging analyses with unmatched precision (funded by the Auvergne-Rhône-Alpes Region, October 2023).
The MALDI-2 mass spectrometer is equipped with two lasers — one for ionization and the other for post-ionization — allowing for deeper sample analysis with enhanced sensitivity. One of the key features of this instrument is its ability to separate isomers upstream of analysis through ion mobility, using PASEF technology (e.g., lipids, bile acids). This enables higher resolution and more precise identification of biological components, even in complex samples.
In addition, it allows for direct analysis of cryopreserved or FFPE tissue sections, enabling in situ localization of biological compounds (intact proteins, lipids, and metabolites) with spatial resolution ranging from 20 down to 5 µm.
The processing and storage of LC-ESI-IM-HRMS and MALDI-IMS imaging data are managed by the platform in close collaboration with the chemometrics and modeling platform — CHEMOD of the ISA.
Platform equipment:
- Cryostat CM3050S (LEICA)
- HTX M3+ sprayer (HTX Technologies, LLC)
- HTX SubliMATE (HTX Technologies, LLC)
- DM12000 fluorescence microscope (LEICA)
- Nano-flow liquid chromatography system (nanoElute 2)
- UHPLC liquid chromatography system (Elute 2)
- timsTOF fleX MALDI-2 mass spectrometer with microgrid option (5 µm spatial resolution)
Our supporters: Auvergne-Rhône-Alpes Region, UCBL, CNRS Chemistry (Emergence@Int.)
Our partners: AmberGen, Bruker
Collaborations: Toronto General Hospital Research Institute (Toronto Western Hospital Liver Clinic, UHN), Saint Louis University – Department of Molecular Microbiology and Immunology, INSERM UMR 1350 PathLiv, INSERM UMR 1060 CarMeN Laboratory Lyon, Department of General, Digestive and Hepatic-Intestinal Transplant Surgery (HCL Croix-Rousse), Department of Pediatric Visceral, Thoracic and Transplant Surgery (HFME).
For any quote requests, please contact us at the following address: sophie.ayciriex@univ-lyon1.fr
Contacts:
Sophie Ayciriex (Associate Professor, HDR, UCBL)
Administrative and Scientific Manager of the Platform
Yohann Clément (IR UCBL)
Co-scientific manager of the platform – Data Management
Justine Massias (Contract Research Engineer, UCBL)
Technical Manager of the Platform
